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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: By-Passing Large Screening Experiments Using Sequencing as a Tool to Identify scFv Fragments Targeting Atherosclerotic Lesions in a Novel In Vivo Phage Display Selection
doi: 10.3390/ijms13066902
Figure Lengend Snippet: Immunodetection of scFvs in sections of vulnerable atherosclerotic lesions from atherosclerotic rabbits. Selected scFvs and irrelevant control scFv (random CDRH3 and CDRL3) were incubated with rabbit tissue sections. After addition of secondary antibodies, sections were treated with the DAB substrate kit reagent. The presence of the antigen recognized by scFvs was indicated by a yellow-brown stain. Incubation with B2.31.F1 ( panel 1 , A , B ), H8.32.F1 ( panel 1 , C ) and A5.31.F1 ( panel 1 , D , E ) scFvs originating from the F1 fraction, F8.32.F2 ( panel 2 , A , B ), C4.31.F2 ( panel 2 , C ) and E1.31.F2 ( panel 2 , D , E ) scFvs originating from the F2 fraction and A2.32.F3 scFv ( panel 3 , A , B ) originating from the F3 fraction revealed the specific localization of each binding. Foam cell-rich advanced atherosclerotic lesions were stained with RAM-11 mAb (positive control) ( panel 1 , G , H; panel 2 , G , H ), whereas incubation with an irrelevant scFv produced no staining ( panel 1 , F , I; panel 2 , F , I ). No staining was observed in the presence of secondary antibody only ( panel 3 , C ). L = artery lumen; I = intima; M = media; VV = vasa vasorum; NC = necrotic core; FC = foam cell; EC = endothelial cell; CH = cholesterol, Ad: Adventitia. Scale bars represent 100 μm.
Article Snippet: Bound secondary antibody was detected with the
Techniques: Immunodetection, Incubation, Staining, Binding Assay, Positive Control, Produced